Plasmid

Part:BBa_K1160001:Design

Designed by: Yang Rongwu   Group: iGEM13_NJU_NJUT_China   (2013-09-14)


chimeric RNA，combining crRNA and tracrRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 249
    Illegal XbaI site found at 1427
    Illegal SpeI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 249
    Illegal BglII site found at 1412
    Illegal BamHI site found at 969
    Illegal BamHI site found at 1689
    Illegal XhoI site found at 260
    Illegal XhoI site found at 450
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 249
    Illegal XbaI site found at 1427
    Illegal SpeI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 249
    Illegal XbaI site found at 1427
    Illegal SpeI site found at 712
    Illegal NgoMIV site found at 1442
    Illegal NgoMIV site found at 1813
    Illegal NgoMIV site found at 3882
    Illegal AgeI site found at 137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 4895
    Illegal BsaI.rc site found at 143
    Illegal BsaI.rc site found at 3988
    Illegal SapI site found at 1822


Design Notes

Source

The DNA sequence corresponding to crRNA is from the type I CRISPR system in E.coli stri. k12, substr. W3110.

References

W3110 genome, NCBI, AC number: NC_007779

Spacers and Repeats information: http://crispr.u-psud.fr/crispr/crispr.php